Welcome to your source for info about cisTEM

cisTEM (computational imaging system for Transmission Electron Microscopy) n -

cisTEM is user-friendly software to process cryo-EM images of macromolecular complexes and obtain high-resolution 3D reconstructions from them. It was originally developed by Tim Grant, Alexis Rohou and Nikolaus Grigorieff and comprises a number of tools to process image data including movies, micrographs and stacks of single-particle images, implementing a complete “pipeline” of processing steps to obtain high-resolution single-particle reconstructions. cisTEM is distributed under the Janelia Research Campus Software License and can be downloaded here.

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Beyond a single pipeline

The main objective of this documention is to enable you to use and/or contribute to the image processing library underlying cisTEM.

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